Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOSL2

Cell type

Cell type Class
Epidermis
Cell type
SK-MEL-147
NA
NA

Attributes by original data submitter

Sample

source_name
SKmel147 ARID2 KO
construct
Lenti-Cas9 + PLKO sgRNA stable infection
disease state
metastatic melanoma
melanoma molecular subtype
NRASQ61R
antibody
FOSL2 - Santa Cruz #sc-604

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
XL ChIP-seq: For ARID2, SS18, BRG1, TEAD4, FOSL2, CTCF, and MITF cells were cross-linked with 2 mM disuccinimidyl glutarate (DSG, Pierce 20593) in PBS for 45 min followed by 1% FA for 10 min at RT. For H3K27ac and H3K27me3 cells were cross-linked with 1% FA for 10 min at RT. ChIP was performed as previously described (Fontanals-Cirera B, Hasson D, Vardabasso C, Di Micco R et al. Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene. Mol Cell 2017 Nov 16;68(4):731-744.e9) ChIP-seq: Libraries for ChIP-seq were done as previously described (Fontanals-Cirera B, Hasson D, Vardabasso C, Di Micco R et al. Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene. Mol Cell 2017 Nov 16;68(4):731-744.e9) ATAC-seq: For all ATAC-seq libraries, 50k cells were harvested, tagmented with 2.5-5uL Nextera Tn5 Transposes from nextera for 30 min, amplified up to 10 cycles and purified essentially as previously described in ATAC protocols. Purified libraries were then size selected on 2% agarose gel (150-700bp).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
63213593
Reads aligned (%)
64.4
Duplicates removed (%)
9.9
Number of peaks
72075 (qval < 1E-05)

hg19

Number of total reads
63213593
Reads aligned (%)
64.1
Duplicates removed (%)
11.2
Number of peaks
71695 (qval < 1E-05)

Base call quality data from DBCLS SRA